Journal: Nature Communications

Article Title: Membrane cholesterol access into a G-protein-coupled receptor

doi: 10.1038/ncomms14505

Figure Lengend Snippet: Control (closed circles) and 5 mM MβCD (40 min) (open circles) treated cells were incubated with different concentrations of [ 3 H]ZM241385 as described in the Methods. These results are mean±s.e.m. values obtained from six separate experiments carried out in duplicate. Kinetic parameters ( B max and K d ) of the corresponding saturation binding curves are indicated at the bottom the figure. ** P <0.01 significantly different from control value according to a Student's t -test.

Article Snippet: The binding data were analysed using Student's t -test, one-way analysis of variance and nonlinear regression fitting to saturation: or competition: binding curves with the GraphPad Prism 5.0 program (GraphPad Software, San Diego, CA, USA).

Techniques: Control, Incubation, Binding Assay